Journal: Cancer Management and Research

Article Title:

MiRNA-128 and MiRNA-142 Regulate Tumorigenesis and EMT in Oral Squamous Cell Carcinoma Through HOXA10

doi: 10.2147/cmar.s250093

Figure Lengend Snippet: Figure 1 MiR-128 and miR-142 are downregulated in OSCC tissues and cells. (A and B) RT-qPCR assay was implemented to detect the expression profiles of miR-128 and miR-142 in 60 pairs of OSCC tissues and adjacent nontumor tissues. (C and D) Expression levels of miR-128 and miR-142 in OSCC cell lines (SCC-9 and SSC-25) and normal Human Oral Keratinocyte cell line (NOK) were measured by RT-qPCR. *P <0.05.

Article Snippet: Human OSCC cell lines (SCC-9 and SSC-25) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Quantitative RT-PCR, Expressing

Journal: Cancer Management and Research

Article Title:

MiRNA-128 and MiRNA-142 Regulate Tumorigenesis and EMT in Oral Squamous Cell Carcinoma Through HOXA10

doi: 10.2147/cmar.s250093

Figure Lengend Snippet: Figure 2 MiR-128 and miR-142 suppress proliferation, migration and invasion, while induce apoptosis in OSCC cells. SCC-9 and SSC-25 cells were transfected with miR- 128, miR-142 or miR-control. (A and B) Transfection efficiency of mature miR-128 or miR-142 in SCC-9 and SSC-25 cells was determined by RT-qPCR. (C) Proliferation of transfected SCC-9 and SSC-25 cells was tested by MTT assay. (D) The expression level of proliferation-related protein ki-67 was detected by Western blot assays. (E) Apoptosis analysis in mature miR-128 or miR-142-transfected SCC-9 and SSC-25 cells was conducted by flow cytometry. (F) Levels of EMT-related proteins (E-cadherin, N-cadherin and Vimentin) were detected by Western blot assay in transfected SCC-9 and SSC-25 cells. (G and H) Cell migration and invasion in mature miR-128 or miR- 142-transfected SCC-9 and SSC-25 cells were analyzed via Transwell assay. *P <0.05.

Article Snippet: Human OSCC cell lines (SCC-9 and SSC-25) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Migration, Transfection, Control, Quantitative RT-PCR, MTT Assay, Expressing, Western Blot, Flow Cytometry, Transwell Assay

Journal: Cancer Management and Research

Article Title:

MiRNA-128 and MiRNA-142 Regulate Tumorigenesis and EMT in Oral Squamous Cell Carcinoma Through HOXA10

doi: 10.2147/cmar.s250093

Figure Lengend Snippet: Figure 4 Silenced HOXA10 curbs proliferation, migration and invasion, whereas boosts apoptosis in OSCC cells. (A and B) HOXA10 expression level was evaluated by RT- qPCR and Western blot assays. (C–K) SCC-9 and SSC-25 cells were transfected with si-HOXA10 or si-control. (C and D) Knockdown efficiency of si-HOXA10 in SCC-9 and SSC-25 cells was determined by RT-qPCR and Western blot assays. (E) Cell proliferation of transfected SCC-9 and SSC-25 cells was examined by MTT assay. (F) The protein level of ki-67 was detected by Western blot assays. (G) Cell apoptosis of transfected SCC-9 and SSC-25 cells was evaluated through flow cytometry. (H and I) Expression of EMT-related proteins (E-cadherin, N-cadherin and Vimentin) in transfected SCC-9 and SSC-25 cells was measured by Western blot assay. (J and K) Migration and invasion of transfected SCC-9 and SSC-25 cells were measured through Transwell assay. *P <0.05.

Article Snippet: Human OSCC cell lines (SCC-9 and SSC-25) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Migration, Expressing, Quantitative RT-PCR, Western Blot, Transfection, Control, Knockdown, MTT Assay, Flow Cytometry, Transwell Assay

Journal: Cancer Management and Research

Article Title:

MiRNA-128 and MiRNA-142 Regulate Tumorigenesis and EMT in Oral Squamous Cell Carcinoma Through HOXA10

doi: 10.2147/cmar.s250093

Figure Lengend Snippet: Figure 6 HOXA10 partially rescued the effects of miR-128 and miR-142 in OSCC cells. SCC-9 and SCC-25 cells were transfected with miR-control, miR-128, miR-128 and vector, miR-128 and HOXA10, miR-142, miR-142 and vector or miR-142 and HOXA10. (A and B) The cell proliferation was detected by MTT assay and Western blot assay in SCC-9 and SCC-25 cell lines. (C) The expression of proliferation-related protein of transfected SCC-9 and SCC-25 cells was assessed by Western blot assay. (D) Cell apoptosis of transfected SCC-9 and SSC-25 cells was evaluated through flow cytometry. (E and F) The protein expression of EMT-related protein E-cadherin, N-cadherin and Vimentin in transfected SCC-9 and SSC-25 cells was measured by Western blot assay. (G and H) The ability of cell migration and invasion were measured by transwell assay in OSCC cells. *P <0.05.

Article Snippet: Human OSCC cell lines (SCC-9 and SSC-25) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Transfection, Control, Plasmid Preparation, MTT Assay, Western Blot, Expressing, Flow Cytometry, Migration, Transwell Assay

Journal: Bioengineered

Article Title: Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2)

doi: 10.1080/21655979.2022.2051886

Figure Lengend Snippet: SHMT2 is up-regulated in OSCC cells. (a-b) The detection of SHMT2 mRNA and protein levels employed RT-qPCR and western blot in OSCC cell lines. *P < 0.05, ***P < 0.001 vs. HOK.

Article Snippet: Normal human oral epithelial cell line (HOK) and several human OSCC cell lines (HN4, SCC-9, and CAL-27) were obtained from BioVector NTCC Inc. HN6 cells were provided by National Institutes of Health of the United States of America.

Techniques: Quantitative RT-PCR, Western Blot

Journal: Bioengineered

Article Title: Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2)

doi: 10.1080/21655979.2022.2051886

Figure Lengend Snippet: ILF2 binds to SHMT2 and can be downregulated by SHMT2 silencing in OSCC cells. (a-b) The interaction between SHMT2 and ILF2 was found through the MINT and BioGRID databases. (c) ILF2 level in oral tumor tissue was analyzed using TNMplot database. (d-e) RT-qPCR and western blot analysis of ILF2 expression. **P < 0.01, ***P < 0.001 vs. HOK. (f) ILF2 expression in SHMT2-knockdown CAL-27 cells was detected with the use of western blot. ***P < 0.001 vs. si-NC. (g) The interaction of SHMT2 and ILF2 was identified using Co-IP.

Article Snippet: Normal human oral epithelial cell line (HOK) and several human OSCC cell lines (HN4, SCC-9, and CAL-27) were obtained from BioVector NTCC Inc. HN6 cells were provided by National Institutes of Health of the United States of America.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Knockdown, Co-Immunoprecipitation Assay

Journal: Bioengineered

Article Title: Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2)

doi: 10.1080/21655979.2022.2051886

Figure Lengend Snippet: ILF2 upregulation reverses the impacts of SHMT2 knockdown on the occurrence of OSCC. (a-b) RT-qPCR and western blot analysis of the overexpression efficiency of ILF2. ***P < 0.001 vs. Oe-NC. (c) CCK-8 assay appraised cell viability in CAL-27 cells transfected with si-SHMT2 and Oe-ILF2. (d) Cell proliferation was identified utilizing colony formation assay in CAL-27 cells transfected with si-SHMT2 and Oe-ILF2. (e-f) TUNEL assay appraised cell apoptosis. (g-h) Western blot analysis of the expression of apoptosis-related factors. **P < 0.01, ***P < 0.001 vs. si-NC; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. si-SHMT2+ Oe-NC.

Article Snippet: Normal human oral epithelial cell line (HOK) and several human OSCC cell lines (HN4, SCC-9, and CAL-27) were obtained from BioVector NTCC Inc. HN6 cells were provided by National Institutes of Health of the United States of America.

Techniques: Knockdown, Quantitative RT-PCR, Western Blot, Over Expression, CCK-8 Assay, Transfection, Colony Assay, TUNEL Assay, Expressing

Journal: Bioengineered

Article Title: Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2)

doi: 10.1080/21655979.2022.2051886

Figure Lengend Snippet: ILF2 elevation restores the impacts of SHMT2 knockdown on OSCC cell migration, invasion and EMT. (a-b) Wound healing and transwell assays appraised cell migration and invasion. (c) Western blot analysis of MMP2 and MMP9 expression. (d) Western blot analysis of the expression of EMT-related factors. ***P < 0.001 vs. si-NC; ## P < 0.01, ### P < 0.001 vs. si-SHMT2+ Oe-NC.

Article Snippet: Normal human oral epithelial cell line (HOK) and several human OSCC cell lines (HN4, SCC-9, and CAL-27) were obtained from BioVector NTCC Inc. HN6 cells were provided by National Institutes of Health of the United States of America.

Techniques: Knockdown, Migration, Western Blot, Expressing

Journal: Oncology Research

Article Title: Fibroblast activation protein (FAP) as a prognostic biomarker in multiple tumors and its therapeutic potential in head and neck squamous cell carcinoma

doi: 10.32604/or.2024.046965

Figure Lengend Snippet: Overexpression of FAP was associated with poorer prognosis in HNSC patients. (a) The expression of FAP mRNA in 40 pairs of HNSC tissues and adjacent normal tissues. (b) Univariate analysis of FAP expression and also clinicopathological factors in TCGA patients with HNSC. (c) Multifactorial analysis of FAP expression and clinicopathological factors in TCGA patients with HNSC. (d) Nomogram for prognostic prediction. (e) The ROC curve of the nomogram predicting survival prognosis.

Article Snippet: The Human oral keratinocytes (HOK) and HNSC cell lines SCC9, SCC15, HN4, SCC25, and CAL27 were provided by the CCTCC.

Techniques: Over Expression, Expressing

Journal: Oncology Research

Article Title: Fibroblast activation protein (FAP) as a prognostic biomarker in multiple tumors and its therapeutic potential in head and neck squamous cell carcinoma

doi: 10.32604/or.2024.046965

Figure Lengend Snippet: FAP mediated the PI3K-Akt signal pathway in HNSC cells. (a) The expression of FAP mRNA in HOK cell line and HNSCs cell lines (SCC15, CAL27, SCC9, HN4, and SCC25). (b) Western blotting was used to detect the expression of FAP protein in HOK and HNSC cells. (c) Western blot analysis of the expression of FAP and PI3K-Akt-related molecules with FAP overexpression or knock-down in HNSC cells. *** p < 0.001.

Article Snippet: The Human oral keratinocytes (HOK) and HNSC cell lines SCC9, SCC15, HN4, SCC25, and CAL27 were provided by the CCTCC.

Techniques: Expressing, Western Blot, Over Expression, Knockdown

Journal: Oncology Research

Article Title: Fibroblast activation protein (FAP) as a prognostic biomarker in multiple tumors and its therapeutic potential in head and neck squamous cell carcinoma

doi: 10.32604/or.2024.046965

Figure Lengend Snippet: FAP overexpression promote the proliferation, migration and invasion of HNSC cells. (a) The results of real-time PCR showing FAP mRNA expression in HN4 cell after transfection of FAP knock-down vector for 48 h (*** p < 0.001). (b) FAP knockdown in HN4 cells inhibited the the proliferative ability of tumor cells (** p < 0.01). (c) FAP knockdown in HN4 cells inhibited the migration and invasion of tumor cells (*** p < 0.001). (d) FAP knockdown in HN4 cells inhibited the migration of tumor cells (*** p < 0.001).

Article Snippet: The Human oral keratinocytes (HOK) and HNSC cell lines SCC9, SCC15, HN4, SCC25, and CAL27 were provided by the CCTCC.

Techniques: Over Expression, Migration, Real-time Polymerase Chain Reaction, Expressing, Transfection, Knockdown, Plasmid Preparation

Journal: Oncology Research

Article Title: Fibroblast activation protein (FAP) as a prognostic biomarker in multiple tumors and its therapeutic potential in head and neck squamous cell carcinoma

doi: 10.32604/or.2024.046965

Figure Lengend Snippet: FAP knock-down inhibite the proliferation, migration and invasion of HNSC cells. (a) The expression of FAP mRNA in CAL27 cell after transfection of overexpression vector of FAP for 48 h (*** p < 0.001). (b) Overexpression of FAP in CAL27 cells facilitated the proliferation were detected in tumor cells. (** p < 0.01). (c) Overexpression of FAP in CAL27 cells promoted the migration and invasion of tumor cells (*** p < 0.001). (d) Overexpression of FAP in CAL27 cells facilitated the migration of tumor cells (*** p < 0.001).

Article Snippet: The Human oral keratinocytes (HOK) and HNSC cell lines SCC9, SCC15, HN4, SCC25, and CAL27 were provided by the CCTCC.

Techniques: Knockdown, Migration, Expressing, Transfection, Over Expression, Plasmid Preparation